Many thanks for your reply, I understand your English perfectly please don't apologise
They are in the test tube set up yes and the test tube is about half ful of water so they had a good clean supply of water in there.
It is a young lasius Niger colony with about 22 workers and 6 brood.
I can't tell the exact temperature of the place they were but it was positively not in the minus degrees as it was inside the house on the kitchen window there is never a radiator on in the room so at a guess I would say it reached 3 degrees as the coldest temperature but it's purely a guess.
I have removed the queen ant and inspected her and I'm positive she is dead as her abdomin the biggest back part of her body is not how it was before the winter. I am keeping her in a separate test tube just incase I am very wrong and she's just in a really deep sleep but I think that's my wishful thinking.
I have some further questions
If I kept my worker ants and the brood without a queen for a length of time would they loose there smell of the queen?
Could I then get another queen with workers and put a fine net over the end to stop the new queen and workers escaping and then tape the old test tube with workers and brood to the new test tube with the new queen and left them together unable to get at eachother would the queenless workers take to the new scent of the queen in the opposite test tube? And then after some time add a single ant to the new colony to test if they would be accepted if there was no fighting add a few more daily to make sure there was no fighting untill all workers had joined together and we're working under a new queen ?
Sorry if this is a bit confusing