Hey and
I don't know why people havent replied so i will answer a few of you questions

1. the colony can stay in the test tube for the first year, my brother takes his out of the tube after the colony's first hibernation but after a year should be ok.
2. It is better if they are in a dark space but i wouldn't really be worried about sealing off light to your queen. Just cover up part of the tube so they have a little of light so they can get used to it.
3. You could Give your colony some cocoons to boost them but if you do dont give to many if you have a lone queen just give her a few, maybe 10-20. give her maybe 1 worker at a time, if i boost i only give 2 workers to the queen and 10 cocoons.
4. When you first put them into the tank not much will happen, they will move in darkness at night so dont expect to see much going on.
5. What do you mean by instuction, if you mean how to care for a colony then go to this thread and follow the links........
viewtopic.php?f=27&t=6355&start=06. The key factor factor is not to let there test tube/nest moist and always make sure they have clean water access 24/7.
hope that helps, matt