A couple of weeks ago, I ordered a colony of Pheidole pallidula size 26-50. Sadly the queen arrived in pieces although the rest of the colony was alive . Antstore sent a replacement colony which had only 4 workers in it . As I can’t be bothered to chase Antstore, I decided to attempt introducing the old colony to the new one.
I placed the new colony in an arena to allow space for the workers to run away if things went wrong and began to add workers from the queen less colony. To my surprise, there was no hostility between the workers and the workers form the old colony were even happy to tend to the new queen . A couple of workers later, I decided to add a soldier, as with the previous workers, she was happy to see the new queen. I added another soldier and left them for about ten minutes to settle. Then I decided to add the last soldier to the new queen. This solder was also happy to see a new queen but, very surprisingly, the two soldiers were immediately aggressive and chased the third soldier away . They are from the same colony! I didn’t think that ants would even fight their own sisters to protect a queen and colony they don’t belong to !