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Do the new queens also lay eggs?
Just to give it a shot: If they do, they might be changing from oligogyny to polygyny as time goes by... At least from my opinion they should get the same scent if the live in the same nest...
well they are not laying eggs yet, but they are still being feed and cleaned, im hoping that they should get the same sent but dunno as of yet.
Eretikas: i did not look at what temperature my fridge was at but i would say around 5oC (approx)